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The existing disclosure is directed to some bacterial shipping and delivery car that contains the vector or payload as described herein.
262、细菌噬菌体可选自长尾噬菌体科(非限制性地比如以下属：cam病毒、lika病毒、r4病毒、acadian病毒、cooper病毒、pg1病毒、pipefish病毒、rosebush病毒、brujita病毒、che9c病毒、hawkeye病毒、plot病毒、jersey病毒、k1g病毒、sp31病毒、lmd1病毒、una4病毒、bongo病毒、rey病毒、butters病毒、charlie病毒、redi病毒、baxter病毒、nymphadora病毒、bignuz病毒、fishburne病毒、phayonce病毒、kp36病毒、rogue1病毒、rtp病毒、t1病毒、tls病毒、ab18病毒、amigo病毒、anatole病毒、andromeda病毒、attis病毒、barnyard病毒、bernal13病毒、biseptima病毒、bron病毒、c2病毒、c5病毒、cba181病毒、cbast病毒、ceci病毒、che8病毒、chi病毒、cjw1病毒、corndog病毒、cronus病毒、d3112病毒、d3病毒、decurro病毒、demosthenes病毒、doucette病毒、e125病毒、eiau病毒、ff47病毒、gaia病毒、giles病毒、gordon病毒、gordtnk病毒、harrison病毒、hk578病毒、hk97病毒、jenst病毒、jwx病毒、kellezio病毒、korra病毒、l5病毒、lambda病毒、laroye病毒、liefie病毒、marvin病毒、mudcat病毒、n15病毒、nonag病毒、np1病毒、omega病毒、p12002病毒、p12024病毒、p23病毒、p70病毒、pa6病毒、pamx74病毒、endurance病毒、pbi1病毒、pepy6病毒、pfr1病毒、phic31病毒、phicbk病毒、phieta病毒、phifel病毒、phijl1病毒、pis4a病毒、psa病毒、psimuna病毒、rdjl病毒、rer2病毒、sap6病毒、send513病毒、septima3病毒、seurat病毒、sextaec病毒、sfi11病毒、sfi21dt1病毒、sitara病毒、sk1病毒、slash病毒、smoothie病毒、soups病毒、spbeta病毒、ssp2病毒、t5病毒、tank病毒、tin2病毒、titan病毒、tm4病毒、tp21病毒、tp84病毒、tria病毒、trigintaduo病毒、vegas病毒、vendetta病毒、wbeta病毒、wildcat病毒、wizard病毒、woes病毒、xp10病毒、ydn12病毒和yua病毒)。
In A different distinct embodiment, said specified effect is generating the receiver bacterial cell create, as molecule of interest, transcription factors and/or modified nucleases, especially to activate particular pathways or genes while in the bacteria which can be The natural way turned off.
By “in trans” is meant herein that said protein, peptid, RNA, nucleic acid, molecule or any blend thereof is not really encoded on the exact same nucleic acid molecule as being the just one comprising the origin of replication. In a particular embodiment, mentioned protein, peptid, RNA, nucleic acid, molecule or any mix thereof is encoded over a chromosome or on a plasmid.
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The bacterial colonization stages were being calculated non-invasively by plating dilutions of stool recovered from Every animal independently onto agar plates.
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in the preferred embodiment, the genetic modification is from the 立即贏取 Bacteroides faecis or Bacteroides thetaiotaomicron beta-galactosidase gene. Preferably, the Bacteroides faecis or Bacteroides thetaiotaomicron beta-galactosidase protein With all the genetic modification shows reduced homology with human MYH6 cardiac peptide as compared with the Bacteroides faecis or Bacteroides thetaiotaomicron beta-galactosidase protein with no genetic modification.
The choice of promoter to the vector in accordance with the existing creation can As a result be produced according to the microorganisms to target.
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By “producing the receiver bacterial cell quit producing a offered molecule” is meant herein lessening or abolishing the creation of claimed supplied molecule by claimed bacterial mobile and/or building the receiver bacterial cell create a variant of explained presented molecule.
The focused receiver bacteria may be any bacteria, especially present in an organism, extra particularly inside a mammal organism. it could be any commensal, symbiotic or pathogenic micro organism of the microbiota or microbiome.